PRBB-CRG Sessions Steven Gygi

10/05/202412:00PRBB AuditoriumPRBB-CRG SessionsSteven GygiHarvard Medical School"Proteomics as a biological assay"Host: Eduard SabidóAbstract:Proteomics is the systematic analysis of proteins for their various properties including expression levels, modification state, structure, interaction partners, function, and many more. For protein expression profiling, the combination of mass spectrometry with sample multiplexing creates a unique biological assay where replicates, positive and negative controls, as well as all treatment conditions can be measured in a single experiment. Tandem mass tags (TMT) facilitate these analyses and will soon be available as a 32plex reagent set. In this talk, I will highlight several new advances with these reagents including their use in targeted proteomics assays. As an example, we created an assay to measure short-lived proteins and applied it to 4 human cell lines. We identified ~1000 proteins with targeted degradation resulting in half-lives of only a few hours--often in a cell-line specific manner.